You know I'm actually not sure about that..but since they affect fertility, I would wonder if you would get a good enough sample to bother chilling and shipping anyway. In the ones I have seen, there was a high percentage of dead sperm, proximal droplets, detached heads, tail defects and things like that on the sperm as a result of those types of infections. The samples I"ve actually looked at have been bovine..but there is no reason to believe it would be any different in dogs. I can't see WHY it would not be spread via chilled semen, but if a male is not used for breeding naturally, you greatly reduce the chance of him being infected,... Some forms of mycoplasma don't even require sexual contact to be spread...and many of them influence fertility. Back in the early 90's, I had to treat ALL my dogs for a month because one tested positive for a type of mycoplasma...and there was no point in treating just THAT dog. Cost me a fortune, not to mention being a pain in the ass to walk around every day, 3 x a day, with a tray of liver cookies with pills stuffed in them all and treat 11 dogs. (The drug of choice by culture for that one was tetracycline...and it had to be given 3 x a day.) I never did figure out how I got it, but was showing a lot then and the vet thought maybe at a dog show.